The Real RNA-seq Problem Isn't Sequencing.

Struggling with degraded FFPE RNA? There's a better way.
In this whiteboard video, Covaris walks you through the truCOVER® Total RNA Library Prep Kit — a fast, streamlined workflow designed to deliver reliable whole transcriptome sequencing results even from the most challenging RNA inputs, including severely degraded FFPE samples with DV₂₀₀ as low as 30%.

Whether you are working with archival tumor tissue, fresh frozen samples, or cell lines, truCOVER Total RNA Library Prep gives you:

• 100% detection of known gene fusions and alternatively spliced transcripts — validated against the Seraseq FFPE Tumor Fusion RNA Reference Standard
• Superior performance from low-quality RNA (DV₂₀₀ ≥30%) without workflow optimization
• rRNA depletion to less than 1%, maximizing every sequencing read for informative transcripts
• 30–40% higher proportion of exonic reads vs. leading competitors
• Complete library prep in approximately 4 hours: 40% less hands-on time than traditional RNA library prep methods
• Automation-ready plate-based design: scales from small studies to high-throughput workflows

Powered by Adaptive Focused Acoustics® (AFA®) technology, truCOVER Total RNA uses mechanical RNA fragmentation instead of chemical methods, delivering consistent, unbiased insert sizes regardless of RNA quality or degradation state. No optimization required as DV₂₀₀ changes. No bias introduced by chemical fragmentation conditions.

This makes truCOVER Total RNA Library Prep the ideal solution for:

• FFPE RNA sequencing and archival tissue analysis
• Gene fusion detection in precision oncology workflows
• Alternative splicing event analysis
• Whole transcriptome sequencing (WTS) from degraded samples
• Differential gene expression (DGE) profiling
• Low-input RNA library preparation
• RNA-seq workflows requiring high strand specificity