Leveraging Plate-Based AFA For Robust and Consistent High Throughput Proteomics

Presented by Craig Braun, Co-Founder, IQ Proteomics LLC, Cambridge, MA

Establishing robust and consistent plate-based sample processing workflows compatible with a wide range of sample types is crucial for enabling high-quality, high-throughput proteomics. At IQ Proteomics, we leverage plate-based AFA technology enabled by the Covaris R230 in several components of our proteomics workflows to achieve this goal. AFA lysis and homogenization of cell pellets and mammalian tissues proceeds simply and reproducibly while keeping volumes small and incorporating plate-based sample handling as early in the process as possible. AFA also streamlines and improves downstream bead-based sample cleanup and digestion workflows (SP3). Incorporation of AFA into multiple points of the proteomics sample preparation workflow improves robustness, while increasing throughput. Finally, R230 integration with robotic liquid handling during both lysis and bead-based protein cleanup/digestion enables start to finish automation of proteomics sample prep for high throughput proteomics applications.